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T2 : Peptidomics : identification of mature neuropeptides by tandem mass spectrometry


Objective: To identify mature neuropeptides present in nervous tissues of Eel and Coral.
Experimental design: The successful methodologies developed to analyse the neuropeptidomes of C. gigas and S. officinalis (underway) is applied to investigate the neuropeptidomes of eel and coral. Several extracts is performed from nervous tissues. The organic fractions is analysed by mass spectrometry in nanoLC Maldi-TOF/TOF and in nanoLC ESI-MS/MS. Identification of neuropeptides from MS/MS spectra is performed using the Mascot 2.2.04 (Matrix Science) against appropriate transcriptome or genome databases.