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All experimental protocols for Eel and Cuttlefish comply with the requirements of the European directives (2010/63/UE). Each experimental protocol will be submitted to the ministry of Higher Education and Research for approval via an ethics committee that takes into account the pain that the animal may experience (CENOMEXA C2EA-54 and 0212-02 for cuttlefish and rabbit (immunization), respectively and Cuvier committee C2EA-68 for Eel).